Take That!: "Two Jokers" shows a photo of the deceased third Joker who was once friends with the other two featured in the story, whose design is a clear parody of Jared Leto's version of the character from Suicide Squad (2016). After looking at the picture, Joker #1 quips "Thank God that asshole's dead."

When Poison Ivy's pheromones make Batman and Wonder Woman sexually attracted to each other, Batman's hallucination shows Wonder Woman in a pink filter (with a Sexophone riff and slight Hair Flip included). Overly Narrow Superlative: King Sharks calls Mr. Freeze, “the most woke ice themed villain in New New Gotham”. One memory that Ivy views within her head is her and Harley with Bruce Wayne's credit cards from " Holiday Knights". Tawney's screen crawls provide hilarious commentary on events. For instance, when Dr Psycho shouts the C-Word on live TV for the second time, the crawl reads: "Man says terrible thing again! Will probably get a third chance." When Harley and Ivy find themselves in an awkward, romantically suggestive pose, the crawl reads "Kiss ya? Ive Harley even known ya!" The issue takes up right after the ending of the last episode of the second season of the animated series.Queen of Fables' ridiculous fate of being cursed by Zatanna to live out the rest of her life as a copy of US Tax Code is taken straight from JLA #47. In the comics, she was trapped in that book (instead of the storybook she was originally trapped in) because her power is drawn from imagination, and there is nothing imaginative about a book of tax codes. Secret Identity Apathy: Averted. There's an unmasking of Batman, but Joker hates that it happened. Scarecrow only unmasked Batman to try and be more like Harley but Joker takes it so badly that he melts Scarecrow's head with acid. Villains Out Shopping: A large portion of the show has the villains in utterly inane settings that have nothing to do with super-villainy, like being roommates, talking about their relationships over lunch, having morning coffee together, attending a bar mitzvah, and so on. Pamela Lillian Isley! Don't you ever pull a stunt like that again, do you understand me? I already watched you die once, don't you dare have me do it again! ❞ — Harley Quinn

There is a stigma on female supervillains in this show, according to Poison Ivy and the Queen of Fables, though they could be exaggerating since they're psychotic sociopaths and misogynist . However, an undeniable fact is that the Legion of Doom has only one female member (Cheetah) whom they don't care enough to even remember her name and are a bunch of misogynistic and manipulative males. Deadpan Snarker: A lot of people, but primarily Ivy. You can count the number of lines Ivy has that don't fall straight into this on one hand. Ivy sends some flowers to look for Vixen, and Peaches offers her a ride. Meanwhile, Mephitic decided to conduct some experiments on Harley after realizing that his toxins have no effect on her. Stephanie" is Clayface's airhead student personality in " Riddle U". She's notably worried about her relationship with Chad.The Penguin's nephew Joshua has a bar mitzvah, but Harley ruins the party by killing all the guards at a fake heist. And it turns out the guards were an improv troupe, so nobody cares that they're dead. Later at the same party, Harley tells Joshua that he can't be a man because he's never finger-blasted a girl. In the series, Ivy's full name is Poison GERTRUDE Ivy (" Catwoman"). But here, Harley calls Ivy her full name from the comics, Pamela LILLIAN Isley. Sy suggests calling up his old friend Henry Kissinger for pointers when the gang briefly considers becoming war criminals to get into the Legion of Doom. Hypocrite: While the Legion expels Dr. Psycho from the group for his blatant sexism, this doesn't stop them from continuing their own sexist practices, with no female members of the group shown before Harley is brought in... just so Joker can emotionally manipulate her again; the lot of them leaving the Queen of Fables to rot in a tax book for years; Lex Luthor gaslighting Ivy at the Joker's behest; Scarecrow kidnapping her, drugging her, and stealing her blood; and other terrible behavior.

Reel Torture: In "The Final Joke", Joker tortures Doctor Psycho by strapping him to a chair and forcing him to watch videos of feminist rallies. After challenging the girls to a drinking contest, Constantine is out cold the next morning wearing a shirt that says, "I tanked a shark", a reference to his old fling with King Shark mentioned in Justice League Dark: Apokolips War.Joker's ultimate punishment for Harley is to make her normal again and not actually harm her at all. She considers this such a horrifying idea that she immediately offers to wholeheartedly work for him again and begs him not to go through with it. Yandere: As a child, Harley loved Frankie Muniz so much that she dreamed of kidnapping him, forcing him to marry her, and having his kids. Noodle Incident: Joker apparently "paralyzed" Commissioner Gordon's "partner" sometime before the show, which is why the commissioner has lost it. Before starting their honeymoon, Harley and Ivy go drop Bud and Lou at Selina's, who doesn't know about their intention. GLAAD announces nominees for the 33rd Annual GLAAD Media Awards Spencer Harvey, GLAAD (January 19, 2022).

Adaptation Relationship Overhaul: In this series, Bruce and Selina are romantic with each other like in most other works. Unlike past works, Bruce is the one who's presented as being emotionally clingy, something that can drive a wedge between them. In other works, if any wedge is driven between them, it's usually more of moral/philosophical differences or the law coming between them.Harley wears her classic jester costume from Batman: The Animated Series, but switches out to a new look combining her New 52 outfit with her hairstyle from the Suicide Squad film in the first episode after deciding to dump the Joker for good. She ends up wearing her classic look again in the finale, and she hates it. Adaptational Sexuality: Vixen has a girlfriend in this continuity. Nightfall and Livewire are exes. Apparently Catwoman likes to overshare about her and Batman's love life. When Harley says she wants a nemesis with "hair on his chest" (talking about Robin), Ivy responds that eliminates Batman because Catwoman told her that he waxes everything. Harley uses "impetuous" to describe herself since her kiss with Ivy in " There's No Place to Go But Down". Season 1 ends with a massive earthquake leveling most of Gotham, in a reference to Batman: No Man's Land, the story arc in which Harley debuted in the mainstream comics canon, after previously remaining limited to Animated Series related continuity.