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g citrus peel powder was weighed and dissolved in 10 mL 70% absolute ethanol at a ratio of 1:20 (w/v).
Frontiers | Widely targeted metabolomic profiling combined
SOPs under magnesium stress showed higher contents of flavonoid carbonosides, suggesting their potential for functional food production and as a source of bioactive compounds for medication. The results indicated that magnesium stress had a significant influence on flavonoids at different stages of fruit growth and development.Widely targeted metabolomic profiling combined with transcriptome analysis revealed that flavonoid compounds synthesis was regulated by transcription factors and structural genes during fruit developmental period. Frontiers | Widely targeted metabolomic profiling combined with transcriptome analysis sheds light on flavonoid biosynthesis in sweet orange 'Newhall' (C. Figure7 Weighted gene co-expression network analysis of DEGs related to flavonoid content under magnesium stress in the yellow modules.
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Although the results of qRT-PCR showed good consistency with the transcriptome data ( Supplementary Figure10), future studies are needed to elucidate the function of these genes in flavonoid biosynthesis. In this pathway, phenylalanine is converted into p-coumaryl CoA through a series of reactions catalyzed by PAL, cinnamate 4-hydroxylase (C4H), and 4-coumaroyl CoA ligase (4CL) ( Forkmann and Martens, 2001; Du etal. Overall, these results shed light on the composition of flavonoid compounds in SOPs and highlight the changes in flavonoid metabolism that occur under magnesium stress.
Three AP2/ERF family members modulate flavonoid synthesis by regulating type IV chalcone isomerase in citrus. production areas, soils, and land preparation, 3rd Edition (University of Florida George A Smathers Libraries).
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Navel orange peel hydroethanolic extract, naringin and naringenin have anti-diabetic potentials in type 2 diabetic rats. Magnesium-deficiency-induced alterations of gas exchange, major metabolites and key enzymes differ among roots, and lower and upper leaves of citrus sinensis seedlings.Flavones were the main flavonoid compounds in citrus, and their synthetic structural genes and transcription factors have become the focus of attention. At 90DAF, MS samples were collected from branches without symptoms of magnesium deficiency, while MD samples were collected from branches with leaf yellowing due to magnesium deficiency.
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Comparative metabolomics unravel the effect of magnesium oversupply on tomato fruit quality and associated plant metabolism. In this study, transcriptome analysis was used to uncover the molecular mechanisms governing flavonoid content in SOPs under magnesium stress. Together, these 16 hub genes were considered to be major regulators of flavonoid biosynthesis in SOPs under magnesium stress. Cluster 2 and cluster 3 had the highest proportion of flavones (98) ( Supplementary Figure4), suggesting that these clusters might play a critical role in flavonoid biosynthesis under magnesium stress in SOPs, with flavones significantly affected.The 18 sequencing libraries were named as MS1-1, MS1-2, MS1-3, MS2-1, MS2-2, MS2-3, MS3-1, MS3-2, MS3-3, MD1-1,MD1-2,MD1-3, MD2-1,MD2-2,MD2-3, MD3-1,MD3-2, and MD3-3. According to leaf nutrient classification standards, a Mg content ranging from 200 mg/kg to 290 mg/kg may represent Mg deficiency, and more than 300 mg/kg may represent Mg sufficiency in leaf ( Collins etal. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
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