Schwechheimer, C. et al. Interactions of the COP9 signalosome with the E3 ubiquitin ligase SCF TIRI in mediating auxin response. Science 292, 1379–1382 (2001) Chew, E.-H. & Hagen, T. Substrate-mediated regulation of cullin neddylation. J. Biol. Chem. 282, 17032–17040 (2007)

Simone Cavadini, Eric S. Fischer, Richard D. Bunker, Alessandro Potenza, Gondichatnahalli M. Lingaraju, Weaam I. Mohamed, Mahamadou Faty, Georg Petzold, Wassim Abdulrahman & Nicolas H. Thomä Groisman, R. et al. The ubiquitin ligase activity in the DDB2 and CSA complexes is differentially regulated by the COP9 signalosome in response to DNA damage. Cell 113, 357–367 (2003) The ATI Automotive Technology program prepares mechanics to begin working in the field with its career readiness components.

Scrima, A. et al. Structural basis of UV DNA-damage recognition by the DDB1–DDB2 complex. Cell 135, 1213–1223 (2008) Great Basin offers an Associate of Applied Science and a Certificate of Achievement in Diesel Technology. S.C., E.S.F. and N.H.T, co-led the study. S.C. prepared the specimens for EM data collection and collected EM data with contributions from K.N.G.. S.C. performed EM data processing. E.S.F. developed the binding and activity assays with input from U.H., G.P. and A.P. G.M.L. designed and created CSN mutants with input from S.C. E.S.F, M.F., G.M.L. and W.A. purified proteins for crystallography, cryo-EM and biochemical assays. A.P. and E.S.F. performed functional assays and analysed the results. G.M.L. crystallized CSN. R.D.B. and G.M.L collected the X-ray diffraction data. R.D.B carried out the crystallographic analysis, built the cryo-EM models with input from S.C., and interpreted the results with S.C. and N.H.T.. K.N.G., R.S.P. and H.S. provided access to TEM microscopes and initial training. W.I.M. designed the RBX1–CSN4 fusion construct and purified protein for EM studies. R.E.J.B. and R.B.T. designed and performed the chemical syntheses. S.M. and K.S. performed cellular assays. S.C., E.S.F., R.D.B. and N.H.T. wrote the manuscript with input from G.P. Corresponding author Honda has set up national training centers where mechanics can learn all the intricacies within all makes and models of these vehicles. Lu, G. et al. The myeloma drug lenalidomide promotes the cereblon-dependent destruction of Ikaros proteins. Science 343, 305–309 (2014)

Tang, J. & Chu, G. Xeroderma pigmentosum complementation group E and UV-damaged DNA-binding protein. DNA Repair (Amst.) 1, 601–616 (2002) Waterman, H., Levkowitz, G., Alroy, I. & Yarden, Y. The RING finger of c-Cbl mediates desensitization of the epidermal growth factor receptor. J. Biol. Chem. 274, 22151–22154 (1999) Kucukelbir, A., Sigworth, F. J. & Tagare, H. D. Quantifying the local resolution of cryo-EM density maps. Nature Methods 11, 63–65 (2014)

Specialized Programmatic Accreditation

Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, 02215, Massachusetts, USA Our highly skilled team of technicians at CSN Autos have decades of experience between them, and nearly all of them are dealer-trained. Krönke, J. et al. Lenalidomide causes selective degradation of IKZF1 and IKZF3 in multiple myeloma cells. Science 343, 301–305 (2014) Ciccia, A. & Elledge, S. J. The DNA damage response: making it safe to play with knives. Mol. Cell 40, 179–204 (2010) Our Spur Trading Estate workshop is fully equipped with every tool and machine our technicians need, including genuine ODIS (VAG) diagnostics to find and fix any problem you have with your VW. As a business, we invest continuously in staff training and equipment to keep abreast of the rapid progress and changes in automotive technology.

TMCC features 10 different automotive service programs, ranging from ones that issue skills certificates and an Associate Degree. Auto mechanics have the option of being trained to work on vehicles made by a specific car manufacturer. This can be accomplished by acquiring a specific type of training at a company facility. There are also post-secondary schools who offer this type of instruction. When a mechanic attains these skills, it can lead to a long and prosperous career working on a specific brand of cars. Auto mechanics can choose to specialize in repairing Ford, GM, Honda and other manufacturers. Car dealerships place a premium on auto mechanics who have this skill as their customers can rely on expert technicians to provide service and repair on their vehicles. The state of Nevada is limited in the facilities that offer these programs, although there are some in nearby states and others located on a nation level. Wang, H. et al. Histone H3 and H4 ubiquitylation by the CUL4–DDB–ROC1 ubiquitin ligase facilitates cellular response to DNA damage. Mol. Cell 22, 383–394 (2006)

I centri di riparazione  CSN sono gli unici specialisti di cui hai bisogno per tutte le tue riparazioni in caso di incidente stradale, riparazione di carrozzeria e verniciatura

Brownell, J. E. et al. Substrate-assisted inhibition of ubiquitin-like protein-activating enzymes: the NEDD8 E1 inhibitor MLN4924 forms a NEDD8–AMP mimetic in situ. Mol. Cell 37, 102–111 (2010) Kia has a national training center where mechanics are sent to attain all of the skills and knowledge needed to become an expert in Kia vehicles. Pierce, N. W. et al. Cand1 promotes assembly of new SCF complexes through dynamic exchange of F box proteins. Cell 153, 206–215 (2013) Freemont, P. S., Hanson, I. M. & Trowsdale, J. A novel cysteine-rich sequence motif. Cell 64, 483–484 (1991) Biosignal Research Center, Organization of Advanced Science and Technology, Kobe University, Kobe, 657-8501, Japan