Suzuki, M. T. & Giovannoni, S. J. Bias caused by template annealing in the amplification of mixtures of 16S rRNA genes by PCR. Appl. Environ. Microbiol. 62, 625–630 (1996). MinION has lower read accuracy but can generate much longer read lengths than those from MiSeq. Nygaard et al . 48 analysed building-dust microbiomes using MinION and MiSeq and showed that, at the genus and species levels, MinION reported greater taxonomic resolution than MiSeq. Long reads help alignment-based assignment of taxonomy as well, because of their increasing taxonomical information content. In this study, under the MinION T4 condition, all the environmental samples showed better taxonomic resolution at the genus level than that under MiSeq, the same as previously reported 17, 48. Many papers have been published on software developments and shorter running times with MinION. For example, Kai et al . 38 reported on the possibility of decreasing the sequencing time of MinION by direct PCR approaches and found that a 3-min sequencing run generated a sufficient number of reads for taxonomic assignment and less than two hours was required for identifying appropriate bacterial species.

Lu, Q., Hu, H., Mo, J. & Shu, L. Enhanced amplification of bacterial and fungal DNA using a new type of DNA polymerase. Aust. Plant Pathol. 41, 661–663 (2012). Yatsunenko, T. et al. Human gut microbiome viewed across age and geography. Nature 486, 222–227 (2012). Cai, L. et al. Biased diversity metrics revealed by bacterial 16S pyrotags derived from different primer sets. PLoS ONE 8, e53649 (2013).Ferris, M. J., Muyzer, G. & Ward, D. M. Denaturing gradient gel electrophoresis profiles of 16S rRNA-defined populations inhabiting a hot spring microbial mat community. Appl. Environ. Microbiol. 62, 340–346 (1996). Minion Uno: This is more of a quiet game for later in the party to wind the kids down a little. Each Players take turns playing cards, this is like the original Uno card game just Minion themed. they need to try to get rid of all of their cards. The first player to do so wins the game.

Penna, V. T., Martins, S. A. & Mazzola, P. G. Identification of bacteria in drinking and purified water during the monitoring of a typical water purification system. BMC Public Health 2, 13 (2002).In this talk, we will deep dive into the Minion component and demonstrate how we leverage it in some typical operations tasks. We will also discuss the challenges faced while operating Minion at scale and how we greatly reduced the operational overheads by improving observability and introducing auto-scaling mechanisms. R Core Team. R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. https://www.R-project.org (2018). Quail, M. A. et al. A tale of three next generation sequencing platforms: comparison of Ion Torrent, Pacific Biosciences and Illumina MiSeq sequencers. BMC Genom. 13, 341 (2012). Johnson, J. S. et al. Evaluation of 16S rRNA gene sequencing for species and strain-level microbiome analysis. Nat. Commun. 10, 5029 (2019).

Illumination, Universal, and Gameloft bring you Minion Rush, an endless running game that can be enjoyed offline, anytime! Run through lots of cool locations, dodging devious traps, battling vile villains, and collecting loads of bright, beautiful Bananas! Wu, J. Y. et al. Effects of polymerase, template dilution and cycle number on PCR based 16 S rRNA diversity analysis using the deep sequencing method. BMC Microbiol. 10, 255 (2010). We assure you that your comment has been duly noted and will be passed on to the development team directly. Disclosure: I received a sample of a product to facilitate this post. All views and opinions stated on this post are 100% my own. Liu, L. et al. Comparison of next-generation sequencing systems. J. Biomed. Biotechnol. https://doi.org/10.1155/2012/251364 (2012).

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Schloss, P. D. et al. Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities. Appl. Environ. Microbiol. 75, 7537–7541 (2009). This is one of the few mini-movies that minion(s) are mentioned by name (the other one is Panic in the Mailroom).